Zeiss Axiolab A Operating Manual

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Zeiss Axiolab A Operating Manual Manual pdf 46 page image

Carl Zeiss Axiolab A3-4 B 40-015 e 06/993.2 Illumination and Contrasting TechniquesThe description of the illumination and contrasting techniques is based on the following microscopesettings:• The Axiolab A microscope is ready for operation as described in chapter 2 and switched on asdescribed in section 3.1.• The pushrod (3-2/3) has been pushed in to direct 100% of the light to the observation port of thebinocular tube.3.2.1 Setting of Reflected-Light Brightfield• Place a specimen of as much contrast aspossible (3-2/6) on the specimen stage (3-2/10);if required by the specimen height, lower thespecimen stage via the coarse drive (3-2/9) orsecure it in the lower stop position.• Pull out plane glass pushrod (3-2/2).• Look through the binocular tube (3-2/1) and setthe eyepiece distance and interpupillarydistance again as required (also see section 2.4).• The aperture diaphragm (3-2/4) must be set toapprox. half the size of the opening diameterby turning the adjustment wheel downwards.• Pull out pushrod for luminous-field diaphragm(3-2/5) until stop.• Use the coarse/fine drive (3-2/9, 8) focus thecircular luminous-field diaphragm now visible inthe field of view.• Insert the pushrod for the luminous-fielddiaphragm (3-2/5) again; the entire field of viewis now visible.• Refocusing on the specimen can be performed via the fine drive (3-2/8).• Use the illuminance control (3-2/7) to set the brightness in the field of view to a value agreeable toyour eyes. Depending on the specimen, the contrast is now adjusted via the aperture diaphragm (3-2/4).However, the aperture diaphragm cannot be used to control the image brightness (loss inimage resolution).112 345678910Fig. 3-2 Setting of reflected-light brightfield