Axiolab A Carl ZeissB 40-015 e 06/99 3-133.2.4 Setting of Epi-FluorescenceThe fluorescence technique allows fluorescent structures to be visualized with high contrast in a typicalfluorescence color. The examined material can be autofluorescent or may have been mixed withfluorescent substances (fluorochromes).The epi-fluorescence technique can be performed using equipment for simple fluorescence examinationswith the Axiolab A, together with the 6 V, 30 W halogen illuminator (3-9/1) and the standard brightfieldbeam splitter.The equipment consists of:- a special FL filter slider (3-9/2) which is insertedin the reflected-light illuminator H instead ofthe standard filter slider.- a dia. 25 excitation filter (3-9/3) especially forthe blue excitation of Epodye.- a dia. 25 barrier filter (3-9/5) especially matchedto this excitation filter, which is inserted in oneof the two dust protection sliders (3-9/6). This technique requires much light.Turn the "illuminance" control (3-9/7)to its right stop, fully open aperturediaphragm (3-9/4) via the adjustingwheel.Fig. 3-9 Setting of epi-fluorescence
