Zeiss Axiostar plus Operating Manual

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Zeiss Axiostar plus Operating Manual Manual pdf 16 page image

Instrument descriptionAxiostar plus Objectives Carl Zeiss40-815 e 12/01 1-71.4 ObjectivesThe objectives are the optical centerpiece ofthe microscope.The following is an example of how objectivescan be labeled:CP-ACHROMAT 10×/0.25 ∞/-.where:10× = objective magnification, with a definedcolor ring on the objectiveallocated to each magnification step(Carl Zeiss color code)0.25 = numerical aperture∞ = infinite tube length− = can be used with cover slip thicknessD = 0 or 0.17 mmor0.17 = can be used with cover slip thicknessD = 0.17 mmandOil = oil immersion objectivePh 2 = phase contrast objective with greenobjective labeling and annulardiaphragm Ph 2The objective magnification multiplied by theeyepiece magnification (usually 10×) results inthe visual overall magnification:e. g. 10 × 10 = 100×.The numerical aperture × 1000,e. g. 0.25 × 1000 = 250×, is the highestuseful magnification, i.e. no further details areresolved above that limit.The objective labeling "∞" indicates that theseobjectives may only be used with microscopesfeaturing infinite optics (ICS) and not withinstruments whose objectives are marked with"160" as their mechanical tube length (inmm).The exact observance of the cover slip thicknessof 0.17 mm is all the more necessary thehigher the numerical aperture of theobjective. Therefore, so-called "Corr"objectives can be set for different cover slipthickness via a correction ring. For this, aspecimen area is searched, and the position ofthe correction ring where optimum focus andimage contrast are obtained is determined(refocusing is always required).Immersion objectives are always insensitive todifferences in cover slip thickness.When immersion objectives are used, the airbetween the cover slip and the objective isreplaced with a liquid, which is immersion oilin most cases. The plastic oiler containing20 ml of 581 N Immersol is particularlysuitable for this purpose.Due to their short working distance, objectives25× and higher feature resilient mounts(specimen protection).Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com