Zeiss Axiostar plus Operating Manual

Contents

Zeiss Axiostar plus Operating Manual Manual pdf 63 page image

OperationCarl Zeiss Transmitted-light phase contrast Axiostar plus3-6 B 40-81 e 12/011 Eyelens – auxiliary microscope2 Knurled ring – auxiliary microscope3 Auxiliary microscope4 Binocular tube/phototube5 Hexagonal key SW 26 Centering screws for annular diaphragm centering7 Luminous field diaphragm8 Slider for phase contrast9 Lever for aperture diaphragm10 Phase contrast objective11 DiopterFig. 3-5 Setting transmitted-light phase contrast3.3 Using transmitted-light phasecontrast3.3.1 General principleThe phase contrast technique is ideal forexaminations of thin, unstained specimens, e.g.culture cells.The phase contrast technique uses the opticalmodulators "phase stop and phase ring" andthe interference procedures during theformation of the intermediate image to changethe small phase differences in intensity andcolor differences which are visible to the humaneye.Optimum phase contrast requires a high level ofcleanliness! Therefore, clean the front lens ofthe objective used, the visible condensersurfaces, the upper cover slip surface and thelower carrier plate surface of the specimen withparticular care and carefully remove grease.3.3.2 Transmitted-light phase contrastconfiguration– Abbe condenser 0.9/1.25 and slider forphase contrast, e.g. Ph 2.– When using the Abbe condenser with 5-position turret, set the relevant phase stopposition (Ph1, Ph2 or Ph3) via the turretdisk.– Phase contrast objectives with phase ringsPh 1, Ph 2 or Ph 3 for different averagenumeric apertures which can also be usedin brightfield without any restriction.– The name of the phase stop on the sliderfor phase contrast must correspond to therelevant name on the objective, e.g. Ph 1.Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com