jenway 6850 Operating Manual

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Contents

jenway 6850 Operating Manual Manual pdf 41 page image

41SECTION 9 – DNA/PROTEINThe DNA/Protein measurement mode allows the user to measure multi-wavelength absorbanceratios, such as 260nm/280nm and 260nm/230nm, which are commonly used to estimate a protein ornucleic acid sample’s purity. The mode also includes calculations that can be used to estimate theconcentration of the protein or nucleic acid sample.9.1 DNA MENU OPTIONSThe DNA/Protein measurement mode enablesmeasurement parameters to be changed. The fouroptions at the bottom of the screen allow the user to editthe concentration calculation factors, select themeasurement mode, select the units of themeasurement and reset the modes settings to theirdefault values. These options are selected with thecorresponding function buttons.9.2 ADJUSTING THE MEASUREMENT MODEThe DNA/Protein measurement mode includes two setsof calculations to calculate the concentration of nucleicacid and protein samples. The measurement modeoption can be selected by pressing the function keybelow the Mode option. The options that are availableare:-1. Absorbance Difference 12. Absorbance Difference 2The default equations and factors used by theinstrument are shown below:-Absorbance Difference 1Purity Ratio = Abs@260nm / Abs@280nmDNA Conc (μg/ml) = (Abs@260 x 62.9) – (Abs@280 x 36.0)Protein Conc (μg/ml) = (Abs@280 x 1553) – (Abs@260 x757.3)