jenway 6850 Operating Manual

Also see for 6850: Operating manual

Contents

jenway 6850 Operating Manual Manual pdf 42 page image

42Absorbance Difference 2Purity Ratio = Abs@260nm / Abs@230nmRNA Conc (μg/ml) = (Abs@260 x 49.1) – (Abs@230 x 3.48)Protein Conc (μg/ml) = (Abs@230 x 183) – (Abs@260 x75.8)The option to include the third wavelength as a reference is given after the measurement mode isselected. Use the up and down arrow keys to select Yes or No and press the Enter key to confirm.Selecting the Yes option will result in all the absorbance value measured at wavelength 3 beingsubtracted from the photometric values measured at wavelengths 1 and 2.9.3 WAVELENGTH SELECTIONThe measurement wavelengths of the selected measurement mode can be adjusted by pressing theGOTO key on the instrument keypad. The required wavelengths can then be entered using thenumerical keypad. Press the Enter key to confirm.9.4 ADJUSTING THE CONCENTRATION CALCULATION FACTORSThe measurement mode option can be selected bypressing the function key below the Coeff option.The new concentration calculation factors can beentered using the numeric keypad. Press the Enter keyto confirm.9.5 SELECTING THE UNIT OF MEASUREMENTThe unit of measurement can be selected by pressing the function key below the Unit option. Therequired unit of measurement can then be selected from the available options of IU, mM/L, M/L,μg/mL, mg/mL, mg/L, mEq, ppb, ppm, % and other. Press the Enter key to confirm.