5ContentsSECTION 1 – Introduction.......................................................................................... 81.1 INSTRUMENT DESCRIPTION............................................................................................. 81.2 INSTRUMENT SPECIFICATION.......................................................................................... 8SECTION 2 – Installation ........................................................................................... 92.1 UNPACKING......................................................................................................................... 92.2 INSTALLATION .................................................................................................................... 92.3 DISPLAY ............................................................................................................................. 102.4 CONTROLS ........................................................................................................................ 112.5 REAR PANEL ..................................................................................................................... 132.6 FRONT VIEW ..................................................................................................................... 13SECTION 3 – THEORY AND PRACTICE OF SPECTROSCOPY MEASUREMENTS................................................................................................................................. 143.1 THEORY OF SPECTROSCOPY MEASUREMENT .......................................................... 143.2 NUCLEIC ACID DETERMINATION ................................................................................... 153.3 SPECTROSCOPY MEASUREMENT ................................................................................. 153.4 GOOD PRACTICE GUIDELINES ....................................................................................... 16SECTION 4 – INSTRUMENT SETUP ...................................................................... 184.1 INSTRUMENT NAVIGATION ............................................................................................. 184.2 MAIN MENU SCREEN ....................................................................................................... 184.2.1 Slit Width Setting ................................................................................................................ 184.3 SYSTEM UTILITY MENU ................................................................................................... 194.3.1 Wavelength Reset .............................................................................................................. 194.3.2 Printer Setup ....................................................................................................................... 194.3.3 Lamp Setup......................................................................................................................... 204.3.3.1 Change the Lamp Switch Point .......................................................................................... 204.3.4 Clock Setup......................................................................................................................... 204.3.4.1 Set Time.............................................................................................................................. 204.3.4.2 Set Date .............................................................................................................................. 214.3.4.3 Display Time / Date ............................................................................................................ 214.3.5 Refresh Dark Current .......................................................................................................... 214.3.6 Connect to PC .................................................................................................................... 214.3.7 Beeper On/Off ..................................................................................................................... 214.3.8 Language Selection ............................................................................................................ 214.3.9 Refresh System Baseline ................................................................................................... 214.3.10 Delete Entire Saved Files ................................................................................................... 224.3.11 Restore Default Settings ..................................................................................................... 22SECTION 5 – PHOTOMETRICS ............................................................................. 235.1 PHOTOMETRICS MENU SCREEN ................................................................................... 235.2 METHOD SET UP .............................................................................................................. 235.2.1 Selecting a Wavelength ...................................................................................................... 235.2.2 Selecting the Measurement Mode ...................................................................................... 235.2.3 Selecting the Unit of Measurement .................................................................................... 245.2.4 Entering a Concentration Factor ......................................................................................... 245.3 CALIBRATION .................................................................................................................... 245.3.1 Zero Calibration .................................................................................................................. 245.3.2 Calibrating to a Standard .................................................................................................... 25