Zeiss Axio Examiner Operating Manual

OPERATIONCarl Zeiss Illumination and contrast procedures Axio Examiner58 M60-2-0003 e 05/20124 OPERATIONAxio Examiner research microscopes are offered both in a version for manual operation and in a versionequipped with a motor-driven focusing gear.The basic difference between the two microscope versions is the fact that objective focusing for theAxio Examiner.Z1 is exclusively motor-driven and operated by using the control system and the keypad.No opportunity for manual focusing control is provided directly at that microscope.But all other operator control functions are completely identical between the manual and the motor-driven microscope.4.1 Illumination and contrast procedures4.1.1 Transmitted light bright field according to KÖHLER(1) General operating principleTransmitted-light bright-field microscopy is the most common of all optical microscopy procedures sinceit allows the quick and easy viewing of high-contrast or stained specimens (e.g., blood films).In addition to so-called direct beams, indirect ray beams, i.e., beams diffracted and scattered at thespecimen details, are essential for achieving an image as true to the specimen as possible. The higher theindirect beam proportion (aperture), the higher is the microscopic image's fidelity to the object accordingto ABBE.To make full use of the microscope's and especially of the objective's performance, condenser, luminous-field diaphragm, and aperture stop should be adjusted according to the rules of KÖHLER's principle ofillumination. These fundamental rules for microscope setting will be described in detail in the followingsection on the 4.1.1 (3) "Setting of a transmitted light bright field according to KÖHLER."(2) Transmitted light-bright field equipmentEvery microscope has equipment which allows the performance of the transmitted-light bright-fieldprocedure.All condensers available for delivery may be used for transmitted-light bright fields.