Zeiss Axio Examiner Operating Manual

OPERATIONCarl Zeiss Illumination and contrast procedures Axio Examiner68 M60-2-0003 e 05/2012(3) Switch to reflected-light fluorescenceThe first reflected-light fluorescence setting will beconsiderably easier if you begin with a highlyfluorescent specimen. But demonstrationspecimens may also be used at the beginning.• Switch on the HAL100 halogen lamp.• Switch the condenser turret first to the Hposition for a transmitted-light bright field, andgo the specimen position to be examined.• Keep the optical path in the reflected-lightilluminator blocked using the blocking positionof the reflected-light filter drawer (Figure 4-8/2)for the time being.• Switch on the HBO 100 or HBO 50illuminator (Figure 4-8/1) at the illuminator'stransformer (see section 3.2.3), and allowapprox. 15 min to heat up to operatingtemperature.• Select and switch on the FL P&C reflectormodule with the desired fluorescence filtercombination (depending on the type ofexcitation) at the reflector turret (Figure 4-8/6).• Enable the optical path in the reflected-lightilluminator when using the reflected-light filterdrawer (Figure 4-8/2).• If required, set the illumination intensity of the fluorescent illuminator (HXP/HBO) using the thumbwheel at the FL attenuator (Figure 4-8/3).• Close the luminous-field diaphragm (Figure 4-8/4) until it becomes visible in the visual field.• Turn the two centering screws (Figure 4-8/5) to center the luminous-field diaphragm to the visual fieldborder.• Either open the luminous-field diaphragm until it just disappears behind the visual field border, orreduce the luminous-field diaphragm up and into the visual field if there is a risk that the specimenfades out.• Finally, refocus to the specimen, and optimize the HBO 100 100 / HBO 50 collector position ifrequired. Set the collector to a value at which the visual field illumination appears as uniform aspossible using the shortwave excitation reflector module. No correction of the collector position isrequired any longer for modules with excitation at a longer wavelength.Figure 4-8 Components for reflected-lightfluorescence (Axio Examiner.A1)