Axioskop 2 plus OPERATIONAxioskop 2 mot plus Illumination and contrasting techniques Carl ZeissB 40-075 e 02/01 3-353.3.4.3 Circular polarization contrast(1) ApplicationUnlike polarization contrast, circular polarization contrast does not feature any dark positions whichdepend on the turning angle (azimuth) of the specimen in relation to the polarizer or analyzer. Alltransparent specimens feature their specific interference colors in optical anisotropy.(2) Axioskop 2 plus and Axioskop 2 mot plus configuration− Objectives suitable for polarization− Rotary mechanical stage 75x50/240° R− Circular polarizer D (polarizers must not be integrated in the condenser), including the relevant λ/4-plate− Analyzer module D in reflector turret(3) Setting of Axioskop 2 plus andAxioskop 2 mot plus• Set the microscope as for transmitted-lightbrightfield according to KÖHLER (see section3.3.1 (3)).• Center the rotary stage (3-25/3) (see section2.2.9 (2)) and then remove specimen from thestage.• Swing circular polarizer (3-25/2) in the beampath.• Swing analyzer module D on reflector turret(3-25/4). The field of view now appears dark onaccount of the crossed polarizers.• Push the upper λ/4-plate (3-25/1) into thecompartment for compensators above thenosepiece until stop. The previously dark field ofview becomes bright again.• Turn the λ/4-plate of the circular polarizer(3-25/2) until optimum darkness of the field ofview is achieved. Close the aperture diaphragmby approx. 2/3 of its diameter to increase thedark position.• Position the specimen to be examined on themechanical stage again.1020304050607080901002Fig. 3-25 Components for transmitted-lightpolarization on the Axioskop 2 plus
