OPERATION Axioskop 2 plusCarl Zeiss Quantitative microscopy Axioskop 2 mot plus3-50 B 40-075 e 02/013.7 Quantitative microscopy3.7.1 Measurement of lengthsThe measurement of lengths using the Axioskop 2 plus / Axioskop 2 mot plus requires the following, forexample:− stage micrometer, positive 5 + 100/100 y, D = 0.17 mm as the object− eyepiece crossline micrometer 10:100, d = 26 mm in the eyepieceAn overview of available stage micrometers and eyepiece reticles is given in section 1.7.Before length measurement using the microscope can be performed, the micrometer or scale value of theobjective / eyepiece reticle combination must be determined. This scale value is exactly that distance inthe specimen which complies to one interval of the used crossline micrometer.For calibration, align the scales of the stage micrometer and the crossline micrometer parallel to eachother by turning the eyepiece, and make the zero line of both scales exactly congruent. If, for example,99 increments (of 10 μm each) of the stage micrometer correspond to exactly 100 increments of thecrossline micrometer, as in Fig. 3-32, the resulting scale value k’ for the used objective / eyepiece reticlecombination (A-Plan 10x/0.25 and crossline micrometer 10:100) isk' = x 10 μm = 9.9 μm☞ The distance to be measured should be ≥ 5 mm in the eyepiece field to keep the influence ofrandom measuring deviations as low as possible. Other measuring errors can occur if theeyepiece has not been inserted into the tube until stop.99100
