Zeiss Axioskop 2 plus Operating Manual

Contents

OPERATION Axioskop 2 plusCarl Zeiss Illumination and contrasting techniques Axioskop 2 mot plus3-24 B 40-075 e 02/013.3 Illumination and contrasting techniques3.3.1 Setting of transmitted-light brightfield according to KÖHLER(1) General principleTransmitted-light brightfield microscopy is the most usual of all the optical techniques, since it allows theeasy and fast viewing of high-contrast or stained specimens (e.g. blood smears).For as true-to-object imaging as possible, indirect ray bundles, i.e. ray bundles diffracted and scattered onthe specimen details, are of major importance in addition to the so-called direct ray bundles. The higherthe portion of indirect rays (aperture), the more realistic the microscope image will be, according toABBE's rule.To utilize the entire optical performance of the microscope, especially of the objective, the condenser, theluminous-field diaphragm and the aperture diaphragm should be set in accordance with the rules forKÖHLER illumination. These basic rules of microscope setting are described in detail in section 3.3.1 (3)"Transmitted-light brightfield settings according to KÖHLER on the manual Axioskop 2 plus“.(2) Transmitted-light brightfield equipment of the manual Axioskop 2 plusEach manual Axioskop 2 plus configuration permits performance of the transmitted-light brightfieldtechnique.(3) Transmitted-light brightfield settings according to KÖHLER on the manualAxioskop 2 plus− The manual Axioskop 2 plus has been set up as described in chapter 2.− The manual Axioskop 2 plus has been switched on.• Set the image brightness via voltage control (3-17/1) on the microscope stand.• Place a high-contrast specimen on the specimen holder of the mechanical stage.• Swing in front lens of the universal condenser (3-17/3) (for objectives ≥ 10x) and use drive for heightadjustment (3-18/2) to move condenser to the upper stop. The stop must be set in such a way that thespecimen is not touched by the condenser (for setting of condenser stop, see section 3.3.1 (4)).• Switch turret disk of condenser (if available) to position H for brightfield via the knurled ring. If noseparate brightfield position is available, the turret position (I, II, or III) can be chosen which does notcontain a DIC prism. If all three positions are equipped with DIC prisms, switch off the analyzer in thereflector turret.☞ One turret position of the universal condenser should remain empty for highest demands intransmitted-light brightfield.