Zeiss Axiovert 200 Operating Manual

Also see for Axiovert 135M: Operating manual Instructions for use

Contents

OPERATIONAxiovert 200 Illumination and contrasting techniques Carl ZeissB 40-080 e 03/01 3-31• Swing out 3-position filter changer (3-16/14).• Place a high-contrast specimen on the microscope stage (3-16/21).• Match the eyepiece distance (interpupillary distance) to the user's individual interpupillary distance:For this purpose, pull apart or push together the binocular component (3-16/11) of the tube.• Set the zero point of ametropia compensation on the setting ring (3-16/12) of the eyepieces (3-16/13)to the white dot if no eyepiece reticle is available,to the red dot if eyepiece reticle is available.• If required, optimize focus of the selected object detail via the setting ring of the relevant eyepiece forametropia compensation.• Use the coarse / fine focusing drive (3-16/6) to focus on the selected detail of the specimen. If no lightis visible in the eyepieces, check whether light exits the housing of the halogen illuminator. If this isnot the case, switch on the halogen illuminator by pressing the HAL on/off key (3-16/7).• Use the toggle switch (3-16/8) to set the light intensity to a comfortable brightness. Adjustment ismade in small steps when the key is pressed half down (pressure point), and in big steps when thekey is pressed down completely.• Close luminous-field diaphragm (3-16/16) untilit is visible in the field of view, even if not infocus (3-17/A).• Focus on the edge of the luminous fielddiaphragm (3-16/B) by moving the condenser(3-17/17) vertically.• Center (3-16/C) luminous-field diaphragm usingcentering screws (3-17/15 and 18) and open ituntil the edge of the diaphragm just disappearsfrom the field of view (3-17/D).• For aperture diaphragm setting, remove one eyepiece from the eyepiece tube and set aperturediaphragm (3-16/20) to approx. 2/3 of the diameter of the objective exit pupil (3-17/E).Optimum contrast setting is dependent on the respective specimen.• Insert eyepiece again and, if required, refocus on the specimen via fine drive.• Match the light intensity via the toggle switch. The field size and the objective aperture change after every objective change, i.e. the luminous-field diaphragm and aperture diaphragm settings must be repeated to ensure optimum results.Fig. 3-17 Diaphragm settings for KÖHLERillumination in transmitted-lightbrightfield