Zeiss LSM 880 Basic User's Manual

Also see for LSM 880: Operating manual Quick operation manual Quick start guide User guide

17Smart SetupClicking this opens a new window (shown below) where you can select yourfluorophores from a list. If your fluorophores aren’t in the list check their data sheets for theexcitation/emission wavelengths and find an equivalent fluorophore from the list to select. Toeach fluorophore you can also assign a display colour to distinguish the different fluorophores.All images are in black and white, the display colour is for visualization only! Eg: GFP can beshown as purple.Three possible regular confocal configurations are generated with optimization forspeed (Fastest), for Best Signal and a compromise between both (Smartest). Eachconfiguration will display the speed, percentage of emission signal collected and crosstalk, thefluorophores emission spectrum overlap. We recommend using the Smartest configuration.Before acquiring you should confirm the lightpath parameters are correct and theproper lasers were turned on before moving to the Channels and Acquisition Mode tabs.BEWARE pressing apply will OVERWRITE any current Experiment Configuration!Do not overwrite other users’ settings!Add your Fluorophoreshere!You can also choose theirfake display colors.