Zeiss LSM 880 Basic User's Manual

Also see for LSM 880: Operating manual Quick operation manual Quick start guide User guide

22ChannelsHere you can select which tracks to acquire, set the pinhole size, laser power and thedetector’s Master Gain. What you see in this tab varies depending on the Image Setup youmade earlier, meaning you could see from one to four channels in a single track.For Confocal ModeEach track has a single pinhole opening which should be set for 1 Airy Unit (AU) toachieve true confocal images. Therefore, in a multi-channel track not all channels will have anoptimal pinhole.Every channel has its own laser power, in percentage, and master gain. To start youshould set the Master Gain to 700 in every confocal channel, then set laser power to 1-2% andtake a snap. You should then use the Range Indicator, next to the histogram in the displayarea, to see if you need to adjust the laser power. If you are using the full dynamic range, a lotof RED pixels means you are saturating the detector (all max intensity values will be lost) andyou must lower the laser power as you may damage the detector and are losing informationabout your sample, the background should be mostly BLUE if there is no background from themounting media. You should leave Digital Offset at 0 and Digital Gain at 1.0.For Airyscan ModeThe pinhole of each Airyscan track must be higher than 2 AU, if the pinhole size is in REDit’s too small for proper high-resolution imaging.For Airyscan the Master Gain should be set to 800. Again, you should start at 1-2% oflaser power and adjust as needed based on the Range Indicator.If you are using live or continuous for focusing check only one track, preferably that ofyour less sensitive fluorophore.

/ 25

Related manuals for Zeiss LSM 880