Zeiss LSM 880 Basic User's Manual

Also see for LSM 880: Operating manual Quick operation manual Quick start guide User guide

19To make sure you don’t forget anything follow this order when setting up the lightpath:1. First select the laser line(s) you will need, if you haven’t turned them on yet youwill get a prompt to do so; (2)2. Following the beam, you will then have to choose the appropriate Main BeamSplitter for your laser line(s). Eg: Laser Line 488 uses MBS 488; (3)3. Following the lightpath UP, you then must choose between Mirror for ClassicConfocal and Plate for Airyscan (ignore the other options and never selectNone). (4)Here the path splits, follow the beam to the Left for Confocal and Up for Airyscan.Confocal4. Follow the beam (yellow) to select the detector(s) you want for this track. TheCh2 GaAsP is the most sensitive detector, ideal for sensitive or weakfluorophores. The other two are optimal for strong fluorophores, with Ch3detector having an extra sensitivity to the red part of the spectrum; (5)5. You can also identify the fluorophore from the dropdown list (like in SmartSetup) and assign its display colour. If a fluorophore is selected its emissionspectrum will be shown in the graph above; (5/6)6. Adjust the range of detection, either by wavelength numbers or by adjusting thebar below the spectrum. (5/6)Airyscan4. Follow the beam (white) and select the emission filter that works for yourfluorophore(s). Eg: Fluorophores using Laser line 488 should use BP 495-550 +LP570 filter; (7)5. Check the Airyscan detector. (make sure no other detectors are selected andthat Plate is being used.) (8)Fast AiryscanSelect Fast mode and then follow the setup for normal Airyscan.Transmitted LightCheck the T-PMT at the bottom of the window in any track, this works for bothBrightfield and DIC. (9)Empty positions should be left with Plate selected instead of None.Laser -> Optic Components -> Detector