8ObjectivesMagnification1 10x 20x 63xZEISS System EC Plan-NEOFLUAR Plan-APOCHROMAT Plan-APOCHROMATClass Colour and ChromaticCorrectionColour and ChromaticCorrectionColour and ChromaticCorrectionAperture (A) 0.3 0.8 1.4Immersion Air Air OilFree Working Distance(mm) 5.2 0.55 0.19Cover Glass (mm) 0.17 0.17 0.17Contrasting Methods Phase Contrast (PH1) Differential InterferenceContrast (DIC II)DifferentialInterference Contrast(DIC III)Lateral Resolution (XY)@525 nm (μm) 2 0.7 0.2625 0.15Nyquist Sampling (XY)@525 nm (μm) 3 0.28 0.105 0.06Zoom to NyquistSampling (XY) @525 nmwith 512x512 scanformatNumber offield/(magnification*zoom)= Nyquist samplingAxial Resolution (Z)@525 nm (μm) 20.7350.091.4 × 0.525 × 1.3380.641.4 × 0.525 × 1.5151.96Nyquist Sampling (Z)@525 nm (μm) 3 3.2667 0.6146 0.22731Notice that the microscope is equipped with two Eyepieces: HC Plan S 10x/23 Br. M2