Zeiss LSM 880 Basic User's Manual

Also see for LSM 880: Operating manual Quick operation manual Quick start guide User guide

5Suggestion for “Materials and Methods”Confocal Z-series stacks (step size 200 nm) were acquired on a Zeiss LSM 880 pointscanning confocal microscope using the Airyscan detector, a 63x Plan-Apochromat 1.4NA DICoil immersion objective (Zeiss) and the 405 nm, 488 nm and 561 nm laser lines. The Zeiss Zen2.3 (black edition) software was used to control the microscope, adjust spectral detection forthe emission of 4′,6-diamidino-2-phenylindole (DAPI), Green Fluorescent Protein (GFP) andtetramethylrhodamine (TMR) SNAP-tag fluorochromes and for processing of the Airyscan rawimages.Confocal Z-series stacks and tile regions with multiple positions were acquired on a ZeissLSM 880 point scanning confocal microscope using photomultiplier tube detectors (PMTs) anda gallium arsenide phosphide (GaAsP) detector, a 63x Plan-Apochromat 1.4NA DIC oilimmersion objective (Zeiss) and the 405 nm, 488 nm and 561 nm laser lines. The Zeiss Zen 2.3(black edition) software was used to control the microscope and adjust spectral detection forthe emission of 4′,6-diamidino-2-phenylindole (DAPI), Green Fluorescent Protein (GFP) andtetramethylrhodamine (TMR) SNAP-tag fluorochromes.Images were acquired on a Zeiss LSM 880 point scanning confocal microscope controlledwith the Zeiss Zen 2.3 (black edition) software, using a 63x Plan-Apochromat 1.4NA DIC oilimmersion objective (Zeiss), the fluorescence filter sets GFP + TX2 and differential interferencecontrast (DIC) optics.Information to be added to the acknowledgements sectionThis work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE - "Orçamento de Estado"and by european funds from FEDER - "Fundo Europeu de Desenvolvimento Regional”.