Zeiss LSM 5 MP Quick Manual

Also see for LSM 510 Inverted: Operating manual Operating manual User guide User manual Brief Operating Manual

Contents

08/2007 13Settings for track configuration in Channel Mode" Select Channel Mode if necessary (Fig. 15)." Click on the LSM tab (Fig. 14).The Light Path tool displays the selected trackconfiguration which is used for the scan procedure." You can change the settings of this panel using thefollowing function elements:Activation / deactivation of the excitation wavelengths (check box) and setting ofexcitation intensities (slider). If necessary open the Laser Control tool (see above).Selection of the main dichroic beam splitter (HFT) or secondary dichroic beam splitter(NFT) position through selection from the relevant list box.Selection of an emission filter through selection from the relevant list box.Activation / deactivation (via check box) of the selected channel (Ch 1-4, monitor diodeChM, META detectors ChS1-8, transmission ChD) for the scanning procedure andassigning a color to the channel." Select the appropriate filters and activate thechannels." Click the Laser icon to select the laser lines andset the attenuation values (transmission in %) inthe displayed window." For the configuration of the beam path, pleaserefer to the application-specific configurationsdepending on the used dyes and markers andthe existing instrument configuration." In the Imaging Setup tool the Detection Bands& Laser Lines are displayed in a spectral panel(Fig. 16) to visualize the activated laser lines forexcitation (vertical lines) and activated detectionchannels (colored horizontal bars).Fig. 15 Imaging Setup tool for a single track(LSM)Fig. 16 Detection Bands & Laser Lines display