Zeiss LSM 5 MP Quick Manual

Also see for LSM 510 Inverted: Operating manual Operating manual User guide User manual Brief Operating Manual

Contents

08/2007 15Scanning an imageSetting the parameters for scanning" Select the Acquisition Mode tool from the Left Tool Area (Fig. 18)." Select the Frame Size as predefined number of pixels or enter your own values (e.g. 300 x 600) in theAcquisition Mode tool. Click on the Optimal button for calculation of appropriate number of pixelsdepending on objective N.A. and %.The number of pixels influences the image resolution!Note: When using an Axioskop 2 FS MOT, make sure you set the correct objective manually in theAcquisition Mode tool. This ensures correct calibration, calculation of pinhole and Z stackoptimization, etc. The Axioskop 2 FS MOT does not automatically detect the objective lens.Adjusting scan speed" Use the Scan Speed slider in the Acquisition Mode tool (Fig. 18) to adjust the scan speed.A higher speed with averaging results in the best signal-to-noise ratio. Scan speed 8 usually producesgood results. Use speed 6 or 7 for superior images.Choosing the dynamic range" Select the dynamic range 8 or 12 Bit (per pixel) in the Bit Depth pull-down in the Acquisition Modetool (Fig. 18).8 Bit will give 256 gray levels; 12 Bit will give 4096 gray levels. Publication quality images should beacquired using 12 Bit data depth. 12 Bit is also recommended when doing quantitative measurements orwhen imaging low fluorescence intensities.$Fig. 18 Acquisition Mode tool