08/2007 7Setting up a new image document and saving your dataTo create a new image document in an empty image container, click the "Single" or the "Start"button. The new document is immediately presented in the Open Images Area. Remember, anunsaved 2D image in the active image tab will be over-written by a new scan. Multi-dimensional scans orsaved images will never be over-written and a new scan will then automatically create a new imagedocument.Alternatively you can create a new empty image document with the "New" button in the ActionButton area or the "New" function in the File Menu.Acquired data is not automatically saved to disc. Make sure you save your data appropriately and back itup regularly. The ZEN software will ask you if you want to save your unsaved images when you try toclose the application.Note: There is no "image database" any more like in the earlier Zeiss LSM software versions.Fig. 8 New image document in the Open Images Ares