Zeiss LSM 5 MP Quick Manual
Also see for LSM 510 Inverted: Operating manualOperating manualUser guideUser manualBrief Operating Manual
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18 08/2007The scanned image appears in a false-colorpresentation (Fig. 22).If the image is too bright, it appears red on thescreen. Red = saturation (maximum).If the image is not bright enough, it appears blueon the screen. Blue = zero (minimum).Adjusting the laser intensity" Set the Pinhole to 1 Airy Unit (Fig. 23)." Set the Detector Gain high." When the image is saturated, reduce AOTFtransmission in the Laser control section of theChannels Tool (Fig. 23) using the slider toreduce the intensity of the laser light to thespecimen.Adjusting gain and offset" Increase the Amplifier Offset until all bluepixels disappear, and then make it slightlypositive (Fig. 23)." Reduce the Detector Gain until the red pixelsonly just disappear.Fig. 22 Image DisplayFig. 23 Channels tool |
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