Zeiss LSM 5 MP Quick Manual

Also see for LSM 510 Inverted: Operating manual Operating manual User guide User manual Brief Operating Manual

Contents

16 08/2007Setting scan averagingAveraging improves the image by increasing the signal-to-noise ratio. Averaging scans can be carried outline-by-line or frame-by-frame. Frame averaging helps to reduce photo-bleaching, but does not give quiteas smooth of an image." For averaging, select the Line or Frame mode in the Acquisition Mode tool." Select the number of lines or frames to average.Adjusting pinhole size" Select the Channels tool in the Left Tool Area." Set the Pinhole size to 1 (Airy unit) for best compromise between depth discrimination and detectionefficiency.Pinhole adjustment changes the Optical Slice thickness. When collecting multi-channel images, adjustthe pinholes so that each channel has the same Optical Slice thickness. This is important forcolocalization studies.$Fig. 19 Channels tool