Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510Carl Zeiss Performance Features of the LSM 510 LSM 510 META3-8 B 45-0008 e 10/02It is possible to rotate the XY scanning field through 360° and carry out XY scans without having torotate the specimen itself under laser radiation load.Selection of the specimen detail of interest for zooming is fast and convenient, and the zoomed image isautomatically centered. This saves the job of specimen centration with the microscope stage.Using a bi-directional scanning facility will double the scanning rate to approx. 5 frames/sec (at 512 ×512 pixels); if two different laser wavelengths are used for the two scanning directions (wavelength 1 forleft-to-right, and wavelength 2 for right-to-left scanning), two fluorochrome dyes can be viewed anddocumented in a quasi-simultaneous mode. This will absolutely prevent "bleeding".3.4.2 Microscope Equipment of the LSM 510 SystemThe LSM 510 system is equipped either with the inverted Axiovert 200 M BP or SP microscope or withthe upright Axioplan 2 imaging MOT , Axiotron 2 or Axioskop 2 FS mot microscopes.Only the differences from the delivered operating manual "Axiovert 200 M" will be explained here.(1) Standa) The motorized objective nosepiece 5× H DIC is firmly fixed to the stand, where no operating elementscan be found for the nosepiece. Operation will be performed via LSM 5 software control. The"Restriction of the nosepiece height to protect the objectives during motorized objective change" isinactivated. The nosepiece will be moved down automatically before each motorized objective change.b) The reflector mount is motorized and provided with the Axiovert 200 reflector turret. The reflectorturret has 5 positions: One transmitting light position, which is identical to the LSM position, and fourfurther positions for fluorescence filter sets (reflector modules). If you want to use more than fiveconventional fluorescence filter sets, it is advisable to use a further reflector turret. When changing thereflector turret position you must make sure that the turret will click into position, since otherwise theimage area will be cut.c) The stand has a motorized focusing drive (fine coarse). Sensitivity of the focusing drive is adjusted tothe delivered objectives by the manufacturer. If you want to use other objectives, sensitivity andparfocality can be adjusted via the Axioset program.d) The stand features an integrated power supply for the internal motors and stand electronics. Thepower supply can be switched on at the right side of the stand. External power supply units will be usedfor the mercury vapor short arc lamp.