Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

LSM 510 QUICKSTARTLSM 510 META Quick Start in the Expert Mode Carl ZeissB 45-0008 e 10/02 4-21• To further improve image quality you can slowdown the scan speed, allowing more photonsto integrate on the detector, or apply imageaveraging to remove random noise, or acombination of both. These adjustments aremade by clicking on the Mode button on theScan Control window.• Set the Scan Speed in the Speed panel.• Select the Line or Frame average Mode, theMean or Sum average Method and theNumber of averages in the Depth, ScanDirection & Scan Average panel accordinglyby observing your image. The setting average of16 (Number 16) should improve signal / noisedramatically; however, the image acquisitionrate will be slower.• When the image optimization has beenfinished, click on the Single button to generatea single image of the specimen.• If your specimen is sensitive to photobleaching,you can attenuate the laser illumination byclicking on the Channels button in the ScanControl window. At the bottom of the windowyou can set the percentage of laser power(Transmission [%]) for each excitationwavelength. You will probably have to increasethe Detector Gain if you decrease the laserpower. This setting controls the transmissiondegree of the Acousto-Optical Tunable Filters(AOTF).Try to use as low laser light intensity as possible to prevent sample bleaching. For that purposeincrease the detector gain to a value of approximately 800 V.Fig. 4-20 Scan Control window