Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

LSM 510 QUICKSTARTLSM 510 META Quick Start in the Expert Mode Carl ZeissB 45-0008 e 10/02 4-154.3.2.2 Axiovert 200 M• Click on the Acquire button in the toolbar of the Main menu.• Click on the VIS button in the Acquire subordinate toolbar.• Click on the Micro button.− The Axiovert Control window appears onthe screen.• Put the specimen on the stage - make sure thespecimen is mounted securely and flat. Use thesupplied Convallaria specimen at first.You can view the specimen in either fluorescence(reflected light) or transmitted light.• To view the specimen in transmitted light, setthe Reflector Turret position to None andactivate the Transmitted Light panel byclicking on the Transmitted Light button.Select the On button and control the intensityby the slider.• Select an objective with low magnification byclicking in the Objective panel of the AxiovertControl window.• Set the microscope to KÖHLER illuminationmanually (see Axiovert 200 M operatingmanual).• Select the specimen area to be examined bymoving the XY-stage and focus exactly on theselected area.• Close the Axiovert Control window. Click onthe LSM button.4.3.2.3 Axioskop 2 FS MOTFor setting the Axioskop 2 FS MOT , proceed in the same way as described for Axioplan 2 imaging MOT .Since the Axioskop 2 FS MOT is not motorized (except for the z motor drive), all microscope settings haveto be made manually.The Micro button in the Acquire subordinate toolbar of the Main menu is therefore not available.Fig. 4-13 Axiovert Control window