Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

QUICKSTART LSM 510Carl Zeiss Quick Start in the Expert Mode LSM 510 META4-20 B 45-0008 e 10/02Proceed as follows for image optimization:• Click on the Channels button in the ScanControl window.− The Channel Settings panel and theExcitation of Track (...) panel are displayedin the Scan Control window.• Press the Cont. (continuous scan) button onthe right-hand side of the window. This startsthe continuous image acquisition, which can beinterrupted by pressing the Stop button.• Under the Channel Settings panel all buttonsfor each channel you have set up are displayed.Click Ch1 (channel 1 of track 1), for example, ifyou want to adjust the first image displayed inthe split mode window.• Use the Pinhole slider to set the pinholediameter.− The selected pinhole diameter should besmall enough to still allow the Detector Gainsetting and to provide sufficient imageinformation. 1 Airy unit is a decent value toobtain a confocal XY-image (use the 1button).• If required, adjust the pinhole again (see Mainmenu, Maintain subordinate toolbar, Pinholebutton).• Use the Detector Gain slider to set image contrast and brightness. This adjustment is very sensitive.Try using the left and right arrows to make the adjustment instead of dragging the slider bar. Use theShift and Crtl keys for changing to coarse and fine steps.• To adjust the black level (background) use Ampl. Offset.• Also, try adjusting the microscope by manual focusing. Sometimes you will find that there are otherfocal planes within the specimen which are brighter, and therefore the detector gain will need to beturned down.• Once you have optimized a particular channel, you can switch to the next channel required andrepeat the optimization.• As soon as all channels are optimized, click on the Stop button.Fig. 4-19 Scan Control window