START-UPZEISS Mounting standard components Axiolab 560 430037-7444-001 05/20193.1.7.4 Removing the stage clips and mounting the detachable Pol specimen guide• Remove the stage clips (Fig. 3-10/9) from the Pol rotary stage.• Insert the Pol specimen guide (Fig. 3-10/2) with the two cylindrical pins on the underside into the holesprovided (Fig. 3-10/3) and tighten the two clamp screws (Fig. 3-10/1) with an Allen wrench (AF 2).3.1.7.5 Centering the Pol rotary stageWith high-power objectives, centering can be exactonly for one selected objective.All stages are factory-precentered, i.e. whilerotating the stage the specimen feature set to thecenter will remain in the center. If the specimenfeature moves out of the center of the field of view(Fig. 3-11/5) while the stage is being rotated, thestage should be re-centered by following thisprocedure:• The KÖHLER illumination on the microscopemust be adjusted before centering the stage(see section 4.2.1).• Turn the nosepiece to swing the non-centeringobjective mount.• For centering the stage, use a contrastingspecimen and an eyepiece with a crosslinereticle.• Loosen the stage clamping screw (Fig. 3-11/1) and the screw cap on the stage carrier (Fig. 3-11/3).• Rotate the stage to determine the position of maximum offset of the specimen feature (Fig. 3-11/5,origin of arrow) from the center of the eyepiece reticle.• Reset the two centering screws on the stage carrier (Fig. 3-11/2) using an Allen wrench (AF 1.5)(Fig. 3-11/4) to move the specimen detail by half an arrow length in the direction of the crosslinecenter. Check whether the specimen detail moves when the stage is rotated again; repeat theprocedure, if required.NOTEThe Allen wrenches (AF 1.5) are located in the storage compartment at the back of themicroscope stand.• When centering is finished, re-tighten the screw cap (Fig. 3-11/3).Fig. 3-11 Centering the Pol rotary stage