OPERATIONZEISS Illumination and contrast methods in transmitted light Axiolab 590 430037-7444-001 05/2019(3) Configuring the microscope• Configure the microscope as for transmitted light brightfield microscopy using the KÖHLER method(see section 4.2.1 (3)).• Center the Pol rotary stage (Fig. 4-7/1) (see section 3.1.7.5) and objectives (see section 3.1.7.6).• Swing the polarizer (Fig. 4-7/3) into the beam path and position it to 0° if you are using a rotatablepolarizer.• Insert the analyzer slider (Fig. 4-7/2) into the slot for compensators (if the tube does not already havean analyzer). The field of view will appear dark due to the crossed polarizers. With screw-on analyzersin the intermediate plate for tubes, care must be taken to ensure that they are aligned with polarizer D(i.e. crossed position).• Bring the specimen to be examined into the field of view and turn it with the rotary stage. Normally,birefringent (anisotropic) objects will now show the interference color and intensity variations asdescribed above during rotation between crossed polarizers. Optically anisotropic substances mayremain dark when an isotropic direction, e.g. from optically single-axle or double-axle crystals, isoriented parallel to the observation direction.Fig. 4-7 Components for transmitted light polarization
