97Expanded programs6.4 Single Wavelength (absorbance, concentration and transmittancereadings)The Single Wavelength mode can be used in three ways. Forsample readings at a single wavelength, the instrument can beprogrammed to measure the absorbance, % transmittance orconcentration of the analyte.• Absorbance readings: The light absorbed by the sample ismeasured in absorbance units.• % Transmittance measures the percent of the original lightthat passes through the sample and reaches the detector.• Turning on the concentration factor allows selection of aspecific multiplier for converting absorbance readings toconcentration. In a graph of concentration versus theabsorbance, the concentration factor is the slope of the line.6.4.1 Set up Single Wavelength mode1. Press Single Wavelength in the Main Menu.2. Press Options for Parameter Setup.Table 12 Options for Single WavelengthOptions DescriptionMore For further OptionsRecall measurementdata symbolRecall saved measurement data, wavelength scans or time courses, refer to section 5.3,page 43.%Trans/Ext (%Trans/Abs) To switch to % transmittance, concentration or absorbance readingsλWavelength To input the wavelength setting. Use the alphanumeric keypad to enter the reading wavelengths.The entered wavelength must be in the range from 320-110 nm.Timer iconThis functions as a stopwatch. It helps to make sure that the steps of an analysis are correctlytimed (e. g. reaction times, wait times, etc., can be exactly specified). When the specified timehas elapsed, a sound is emitted. The use of the timer has no influence on the reading program.Concentration Factor Multiplication factor for converting absorbance values into concentration values.ConcentrationResolution To select the number of decimal places.
