99Expanded programs6.4.2 Take single wavelength readings (single reading)1. Insert the zero solution cell into the cell compartment.2. Press Zero.Note: The Read key is only active after the zero reading has beencompleted.3. Insert the analysis cell into the cell compartment.4. Press Read.5. For data storage, refer to section 5.3.1, page 43.6.5 Multi Wavelength mode – readings with more than one wavelengthIn the Multi Wavelength mode, absorbance values can bemeasured at up to four wavelengths and the results can bemathematically processed to obtain sums, differences andrelationships.• Absorbance readings: The light absorbed by the sample ismeasured in absorbance units.• % Transmittance measures the percent of the original lightthat passes through the sample and reaches the detector.• Turning on the concentration factor allows selection of aspecific multiplier for converting absorbance readings toconcentration. In a graph of concentration versus theabsorbance, the concentration factor is the slope of the line.Concentration is calculated using a single factor for eachwavelength, which is input by the user.6.5.1 Set the Reading mode at different wavelengthsPress Multi Wavelength in the Main Menu. Press Options forParameter Setup.