CARE, FUSE REPLACEMENT AND SERVICEZEISS Troubleshooting Axioscope 5/7/Vario128 430035-7344-001 03/20185.3 Troubleshooting5.3.1 MicroscopeProblem Cause SolutionShading or brightness irregularitiesin the field of view of themicroscope; the field of view is notfully visible.The vis/phot push-pull rod/shift knobon the photo tube is not in correctfunctional position (in-betweenposition).Move the vis/phot push-pull rod/shiftknob to the correct functional position(end position).Nosepiece with objective is not fullyengaged in its locking position.Engage the nosepiece with theobjective in its locking position.Condenser is not adjusted correctly. Adjust the condenser correctly(adjustment, centering),see p. 87 ff.Aperture diaphragm is not adjustedcorrectly.Adjust the aperture diaphragmcorrectly (centering, opening),see p. 87 ff.Field diaphragm is not adjustedcorrectly.Adjust the field diaphragm correctly(centering, opening),see p. 87 ff.Filter is not correctly inserted in itsslot.Insert the filter correctly.Low resolution and poor contrast. Opening of the aperture diaphragm isnot adjusted correctly.Adjust the opening of the aperturediaphragm according to the 2/3-ruleand the texture of the sample you areusing,see p. 87 ff.Condenser is not focused correctlyand front lens is not connectedcorrectly.Focus the condenser and turn thefront lens correctly on or off,see p. 87 ff.Wrong thickness of the frame glasswhen using a transmitted lightobjective with a frame glass thicknessof 0.17 mm.Use standardized frame glasses with athickness of 0.17 mm.Specimen holder is not insertedcorrectly.Turn the specimen holder over, thesample side shows up.No immersion oil or an unspecifiedimmersion oil is used with immersionobjectives.Use immersion oil 518 N or 518 F byZEISSAir bubbles in the immersion oil. Repeat the oiling procedure with freshoil.Immersion oil on the front lens of adry objective.Clean the lensCorrection setting is not set to theproper thickness of the frame glass.Adjust the correction setting to thecorrect thickness of the frame glassDirt or dust on the optical surfaces ofobjectives, eyepieces, condensers orfilters.Clean the soiled optical component