CARE, FUSE REPLACEMENT AND SERVICEAxioscope 5/7/Vario Troubleshooting ZEISS03/2018 430035-7344-001 129Problem Cause SolutionParfocal performance not good onAxioscope 7The focal plane was adjusted usingthe low-magnification nosepiecewhich has a larger depth of focusthan the high-magnificationnosepiece.Determine the focal plane using thehigh- magnification nosepiece.There is a backlash of Z-axis drive. Adjust the focal plane from the samedirection for all the nosepieces.No light in eyepiece The system is in ECO mode. Turn the Intensity/LM knob clockwiseto wake up the system.The light intensity is too low. Turn the Intensity/LM knob clockwiseto increase the light.The light was turned off by anotherpressing of the respective RL/TLbutton.Press the RL or TL button according tothe corresponding indicator in greencolor.LED connector is loose (when usingbuilt-in LED10 illumination).Unmount the LED10 lamp case fromthe microscope stand, unplug and re-insert the connector to the socket.Check again.The reflector module is incorrectlyinstalled or absent.Check the reflect turret and makesure the correct reflector is in use.The field diaphragm is closed. Check and, if necessary, open thefield diaphragm.XY stage stops at wrong positionafter initialization on Axioscope 7The XY stage initialization failed. Restart the microscope, if the issuestill exists please contact ZEISS Service.Cannot focus on the sample underhigh- magnification nosepiecewith Axioscope 7The Z-axis resolution was notconfigured with the magnification ofnosepiece.Configure the system with correctnosepiece information with MTBConfiguration.Asymmetric image sharpness, e.g.one side is sharp, one is sideblurred.Condenser is not adjusted properly. Re-adjust the condenser,see p. 87 ff.Nosepiece is not engaged in itslocking position.Engage the nosepiece in its lockingposition (click-diaphragm).Sample is not fixed on the mechanicalstage.Insert and fix the sample correctly inthe specimen holder.Distinct focus differences whenchanging the objective.Focusable eyepieces are not adjustedcorrectly.Adjust the focusable eyepiecesaccording to the user’s vision defect,see p. 85.Objective is not screwed in all theway.Screw the objective in to the stop.Tube lens is not mounted, or it ismounted unnecessarily.Mount the tube lens or remove it,according to the situation.