OPERATIONAxioscope 5/7/Vario Illumination and contrast methods in transmitted light … ZEISS03/2018 430035-7344-001 974.2.5 Setting up transmitted light PlasDIC contrast microscopy(1) General principle of operationPlasDIC can be used independently from the material of the specimen holder.The contrast method gives a relief-like image and is especially well suited for thicker objects. The contrastis adjustable. It is possible to contrast the cavities of microtiter plates up to the edge. It is not necessary touse cultivation holders with a glass base.(2) Instrumentation− Abbe condenser with modulator disk and objective-dependent 2 mm slit diaphragm (A-Plan 10xand LD A-Plan 20x) or 4.5 mm slit diaphragm (in all other cases)− Objectives:A-Plan 10x, 20x, 40x;LD A-Plan 20x, 32x, 40x;LD Plan-Neofluar 20x, 40x, 63x− PlasDIC slider depending on the objective− ACR P&C analyzer module for transmitted light and reflector turret /slider or D/A fixed or rotatableanalyzer slider in connection with mounted intermediate plate for analyzer slider 12x46(3) Setting PlasDIC• Fully open the aperture on the condenser.• Place the sample on the stage.• Swing the position with the 2 or 4.5 mm slit diaphragm for PlasDIC of the condenser into the beampath. Increase brightness when switching from brightfield to PlasDIC.• Swing the analyzer module into the beam path or slide analyzer slider in beam path in intermediateplate.• Swing in the objective for PlasDIC.• Slide the PlasDIC slider on the nosepiece into the DIC slit of the objective in use.• Adjust the contrast with the knurled screw of the PlasDIC slider. You can now see the structures inrelief or in pseudo-darkfield. The best result is achieved in the relief mode.
