START-UPAxioscope 5/7/Vario Mounting standard components ZEISS03/2018 430035-7344-001 553.1.10.6 Centering objectives of polarizingmicroscope standsThe nosepiece with 5-positions HF/DF/Pol and1-position HF/DF/DIC is equipped with fivecenterable objective mounts (without DIC slots) aswell as one objective mount with DIC slot (non-centerable). Accordingly, all objectives can becentered relative to the rotary stage.Stage centering is necessary to ensure that aspecimen feature located in the center of the fieldof view does not move out while you are rotatingthe stage. Centering all objectives ensures that thespecimen feature remains in the center of the fieldof view even when the objective is changed.• Before centering the stage, you must adjust theKÖHLER illumination on the microscope (seesection 4.2.1).• For stage centering, use a contrast-richspecimen and an eyepiece with reticle.• First, turn the nosepiece to swing the non-centerable objective mount (mount with DIC slot) into thelight path. Center the rotary stage for the non-centerable objective mount as describedunder 3.1.10.5.• Turn the nosepiece to move a centerable objective mount into the light path.• Rotate the stage to determine the position of maximum offset of the specimen feature (Fig. 3-18/3,origin of arrow) from the center of the eyepiece reticle.• Turn the two centering screws on the nosepiece (Fig. 3-18/1) using one AF 1.5 Allen wrench(Fig. 3-18/2) each to move the specimen feature by half the arrow length towards the reticle center.Rotate the stage again to check whether the specimen feature moves off. Repeat the centeringprocedure, if necessary.• Center the other four objectives in the same manner.NOTETo preserve this centered state it is imperative to change the objectives only by turning thenosepiece by its knurled ring, without touching the objectives.Fig. 3-18 Centering objectives
