PUTTING THE INSTRUMENT INTO OPERATIONCarl Zeiss Mounting Optional Components Axio Vert.A164 431030-7044-001 05/20123.3.3 Changing the Filter Set in the FLP&C Reflector ModuleThe customer can put together and install the filtersets for the FL P&C reflector module individually.Appropriate filter sets or even completelyassembled FL P&C reflector modules can beordered from Carl Zeiss.• Take the FL P&C (Fig. 3-28/3) reflector moduleout of the reflector turret and set it down. (Seealso Section 3.1.9.)• Use the mounting plate (Fig. 3-28/6) from thetool kit to screw out the retaining ring(Fig. 3-28/1).• Turn over the reflector module to let the filter(Fig. 3-28/2 or 5) fall out onto a soft surface.• Insert the barrier filter (emission filter) as shown in (Fig. 3-28/2) and the excitation filter as shown in(Fig. 3-28/5), and then secure them with the retaining rings (Fig. 3-28/1).Both the barrier filter and the excitation filter may carry their designation and an arrow on thecircumference. The arrow, which must always point inwards, indicates the direction in which the filter inquestion is to be installed into the reflector module. (See arrows in Fig. 3-28.)With a view to minimizing the image shift in multifluorescence images, the barrier filter may be providedwith another marking, i.e., a marking that indicates the position of the wedge angle.When installing the barrier filter into the reflector modules used, be sure to align that marking to theorientation groove (Fig. 3-28/4). This ensures that the wedge angle of the barrier filter will be in the samedefined position in the reflector module in use, thus compensating, among modules, for and/orminimizing what is actually a very small image shift anyway in the Zeiss filter sets utilized.Fig. 3-28 Changing a Filter Set in the FL P&CReflector Module
