OPERATIONAxio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss05/2012 431030-7044-001 81Setting DIC• Open the aperture diaphragm on the condenser completely. Put on the specimen.• On the nosepiece, rotate in the objective suitable for DIC.• Put the corresponding DIC slider (Fig. 4-8/3) into the slot on the nosepiece. Make sure the DIC slidersnaps safely into position.• On the condenser, (Fig. 4-8/1) rotate the proper DIC I, II or III condenser module (as identified by thestickers on the front of the condenser) into the light path.• Put the three-position contrast slider (Fig. 4-8/2) with the analyzer position into the stand or rotate inthe D P&C analyzer module on the reflector turret. Watch for the correct locking position.• Adjust optimum contrast, using the knurled thumb screw on the DIC slider (Fig. 4-8/4). By adjustingthe DIC slider symmetrically around its middle position, you can render specimen details spatially asthough they were raised or recessed.Fig. 4-8 Setting DIC
