OPERATIONAxio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss05/2012 431030-7044-001 774.11.2 Transmitted Light Phase ContrastRequirements− The microscope must have properly been putinto operation, as described in Section 3.− The microscope must be switched on.− Condenser with slider or modulator disk forphase contrast− Objective for Ph1, Ph2 or Ph3 phase contrastmatching the diaphragm of the Ph1, Ph2 or Ph3condenserPhase contrast objectives come witha green identification marking.Setting Phase Contrast• Rotate the objective for phase contrast(Fig. 4-3/4) into the light path.• Open the aperture diaphragm (Fig. 4-3/3) of thecondenser completely.• Instead of the eyepiece, insert the auxiliarymicroscope (Fig. 4-3/5) into an eyepiece tube and render the phase rings of the objective visible byfocusing its eye lens.The phase ring of the phase contrast objective appears as a gray ring in the bright pupil (Fig. 4-4/A).• Move the phase stop of the slider (Fig. 4-3/1) into the light path (or, on the condenser with modulator,rotate the position with the phase stop into the light path).The phase ring of the objective will appear centered in the field of view, while the bright phase stop maybe off-center (Fig. 4-4/B).Fig. 4-3 Setting Phase ContrastFig. 4-4 Centering the Phase Ring Diaphragm (Bright in the Condenser) to the Phase Ring (Dark in theObjective)
