OPERATIONAxio Vert.A1 Illumination and Contrast Techniques in Transmitted Light Carl Zeiss05/2012 431030-7044-001 834.11.6 Transmitted Light iHMCiHMC (improved Hoffman modulation contrast) is an optimized version of the HMC contrast technique.It produces a relief image of the specimen viewed. Much like PlasDIC, this type of contrast hasestablished itself as the standard in the IVF (in-vitro fertilization) area, in particular.Requirements− The microscope must have properly been put into operation, as described in Section 3.− The microscope must be switched on.− Condenser 0.4 with modulator disk and built-in iHMC modules (1, 2 or 3)− Polarizer slider D 10 mm x 46 mm, rotatable 90 degrees− Objective for iHMC− Auxiliary microscopeAligning the iHMC Module in the Condenser• Rotate in the condenser (Fig. 4-9/5) in such a way that its change position (Fig. 4-9/4) is located on theright-hand side of the transmitted light illumination carrier (Fig. 4-9/1). For this purpose, turn theclamping ring on the condenser to the left, lift it briefly and then rotate the condenser 90 degrees tothe right until it locks into position.• Rotate the iHMC position of the condenser into the change position (Fig. 4-9/4).Fig. 4-9 Setting Relief Orientation on the iHMCSee Page 61 for the installation of the iHMC module.
