OPERATIONCarl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A186 431030-7044-001 05/20124.11.7 Transmitted Light PolarizationContrastThe technique of viewing transparent objectsbetween crossed polarization filters is employed ingeology and mineralogy, in particular. Increasingly,it is also used for plastic, glass, technical films, etc.With this technique, optical anisotropies (e.g.,tensions) are rendered visible, which wouldotherwise go undetected with reflected ortransmitted light methods.Setting the Polarization on the Axio Vert.A1MAT, Using Polarizer DL and Analyzer Slider A12x35 mm, Rotatable 90 Degrees• Set up the microscope for bright field intransmitted light.• Slide polarizer DL (Fig. 4-12/1) on the condenser(Fig. 4-12/2) in the light path.• Insert the analyzer slider (Fig. 4-13/2) (a P&Canalyzer module in the reflector turret may beused as an alternative) into the slot (Fig. 4-13/1)under the nosepiece. The analyzer can berotated 90 degrees.• Use the analyzer turning lever (Fig. 4-13/4) tocross the vibration direction of the analyzer andpolarizer (dark position).• The analyzer slider may be inserted both fromthe left-hand and right-hand sides.For the purpose of color-contrastinganisotropic materials, compensatorLambda or compensator Lambda Polsub. ±10° (Fig. 4-16/3) may be used.To do so, place the compensator onthe analyzer.The typical approach would be firstto bring in crossed position thepolarizer and analyzer, without thespecimen being in the light path,and to put on the specimen onlywhen the field of view has reachedmaximum darkness.Polarizer DL may only be used together with condenser 0.4 with modulator disk 424228-9010-000.Fig. 4-12 Setting Bright FieldFig. 4-13 Setting the Polarization
