OPERATIONCarl Zeiss Illumination and Contrast Techniques in Transmitted Light Axio Vert.A182 431030-7044-001 05/20124.11.5 Transmitted Light PlasDICPlasDIC, an innovative interference contrast, delivers relief-like images and is to be used for thickerspecimens, in particular.The contrast is variably adjustable. The cavities of microtiter plates can be contrasted right to the edge.Cultivation vessels are not required, but are equally suitable.Requirements− The microscope must have properly been put into operation, as described in Section 3.− The microscope must be switched on.− Condenser with slider for PlasDIC or condenser 0.4 or 0.55 with modulator disk and built-in slitdiaphragm for PlasDIC− PlasDIC slider, dependent on the objective used, for the corresponding objective position in thenosepieceorthree-position contrast slider (10 mm x 29 mm) with built-in LD A-Plan 10x-63x PlasDIC module forthe contrast slider (suitable for LD A-Plan 10x to 63x objectives)− D P&C analyzer module in the reflector turret or three-position contrast slider with built-in analyzer forthe contrast slider (one of the two required if a PlasDIC slider is used)− Objective for PlasDIC (e.g., LD A-Plan 10x, 20x, 32x, 40x or 63x)Setting PlasDIC• Open the aperture diaphragm on the condenser completely. Put on the specimen.• Rotate in the PlasDIC diaphragm on the condenser (Fig. 4-8/1) with modulator disk (or move thePlasDIC position of the slider on the condenser into the light path). Brightness may have to beincreased, as appropriate, when you change from bright field to PlasDIC.• When employing a PlasDIC slider, rotate the D P&C analyzer module of the reflector turret into thelight path or move the three-position contrast slider with the analyzer position (Fig. 4-8/2) into the slotunder the nosepiece. If you use the PlasDIC module with the three-position contrast slider, no analyzerwill be required.• Rotate in the objective for PlasDIC. The following are suitable objectives: LD A-Plan 10x, 20x, 32x, 40xor 63x, LD Plan-Neofluar Korr 20x, 40x or 63x.• On the nosepiece, place the PlasDIC slider (Fig. 4-8/4) into the DIC slot of the objective used or movethe PlasDIC module, sitting in the slider, into the light path.• Set the contrast via the knurled thumb screw of the PlasDIC slider (Fig. 4-8/5) or of the PlasDICmodule. Structures may be shown in relief or in pseudo dark field images. Relief imaging delivers thebest contrast.
