Zeiss Axio Scope.A1 Operating Manual

Contents

OPERATIONAxio Scope.A1 Illumination and Contrasting Method Carl ZeissM60-2-0007 e 05/08 694 OPERATION4.1 Illumination and Contrasting Method4.1.1 Adjusting the Transmitted Light/Bright-Field According to KÖHLER(1) General principle of operationThe transmitted light/bright-field microscopy method is among all optical microscopy methods the onewhich is most commonly used. High-contrast or tinted samples (e.g. a blood smear) can be examinedeasily and quickly.For an imaging result which is as true to the object as possible we need not only consider the so calleddirect bundled beams but just as well the indirect ones, i.e. the beams which diffract and scatter on thesample details. According to ABBE, the image is more true to the object when the fraction of the indirectbundled beams is as larger as possible.The best performance of the microscope, and especially its objective, is achieved when condenser, fielddiaphragm and aperture diaphragm are adjusted according to the KÖHLER illumination principle. Thesefundamental basic rules for adjusting a microscope are explained in detail in chapter 6 0 8 H 6 0 8 H 4.1.1 (3) "Adjustingthe transmitted light/bright-field according to KÖHLER".(2) Instrumentation transmitted light/bright-fieldEvery microscope (except the one with the stand column Vario) is configured to work with thetransmitted light/bright-field method.All available condensers (except special condensers like dark-field condensers) can be used for thetransmitted light/bright-field method.(3) Adjusting the transmitted light/bright-field according to KÖHLER− The Axio Scope.A1 has been put into operation appropriately (chapter 6 0 9 H 6 0 9 H 3).− The Axio Scope.A1 is turned on.• Adjust image brightness with light intensity control (6 1 0 H 6 1 0 H Fig. 4-1/2) on microscope stand.• Put high-contrast sample into object holder of mechanical stage.• Bring front lens on condenser (if in use) into place (in objectives ≥ 10x) and turn the gear knob forvertical adjustment of the condenser (6 1 1 H 6 1 1 H Fig. 4-1/3 and 6 1 2 H 6 1 2 H Fig. 4-2/2) to the top stop. Make sure the stop isadjusted in a way to prevent the condenser from lifting out the sample (adjusting the condenser stop,chapter 6 1 3 H 6 1 3 H 4.1.1 (4)).• On condensers with revolver/modulator disks: turn the knurled ring (6 1 4 H 6 1 4 H Fig. 4-2/3) to position H (bright-field).• Bring objective 10x in place on nosepiece (6 1 5 H 6 1 5 H Fig. 4-1/6) and focus sample with gear knob (616H616HFig. 4-1/1).• Close field diaphragm (6 1 7 H 6 1 7 H Fig. 4-1/5) enough to make it visible in field of view (even if blurred)(6 1 8 H 6 1 8 H Fig. 4-1/A).• Lower condenser with gear knob for vertical adjustment until edge of field diaphragm appears sharp(6 1 9 H 6 1 9 H Fig. 4-1/B).