OPERATIONAxio Scope.A1 Illumination and Contrasting Method Carl ZeissM60-2-0007 e 05/08 75• If the overlap is not exact (632H632HFig. 4-5/A), thelighter annular diaphragm must be recenteredwith the aid of two hexagon screwdriversSW 1.5 (6 3 3 H 6 3 3 H Fig. 4-4/1 and 4). Adjust the twocentering screws (634H634HFig. 4-4/2 and 3) so that youachieve a full overlap with the darker phase ring(6 3 5 H 6 3 5 H Fig. 4-5/B).• Remove the auxiliary microscope from the tubeand replace it with the eyepiece.In order to increase the image contrast, aninterference broadband filter, green 32 x 4, may bemounted on the field diaphragm or inserted intothe color glass carrier (if available).A complete phase contrast can only be achievedwhen the light-colored annular diaphragm (in thecondenser) overlaps exactly with the dark-coloredphase ring (in the object) in the illumination beampath (636H636HFig. 4-5/B).Fig. 4-5 Centering the annular phasediaphragm (light-colored, in thecondenser) and the phase ring(dark-colored, in the object)
