OPERATIONCarl Zeiss Illumination and Contrasting Method Axio Scope.A178 M60-2-0007 e 05/084.1.5 Adjusting PlasDIC-Contrast in Transmitted Light(1) General principle of operationPlasDIC can be used independently from the material of the specimen holder.The contrast method gives a relief-like image and is especially well suited for thicker objects. The contrastis adjustable. It is possible to contrast the cavities of microtiter plates up to the edge. It is not necessary touse cultivation holders with a glass base.(2) Instrumentation− Abbe condenser with modulator disk and objective-dependent slit diaphragm 2 mm (A-Plan 10xand LD A-Plan 20x) or slit diaphragm 4.5 mm (in all other cases)− Objectives:A-Plan 10x, 20x, 40x;LD A-Plan 20x, 32x, 40x;LD Plan-Neofluar 20x, 40x, 63x− Slider PlasDIC depending on the objective− Analyzer module ACR P&C for transmitted light and reflector turret /slider or analyzer slider D/Afixated or rotatable in connection with mounted intermediate plate for analyzer slider 12x46(3) Adjusting PlasDIC• Fully open the aperture on the condenser.• Put down the sample.• Swing into the beam path the position with the slit diaphragm 2 or 4.5 mm for PlasDIC of thecondenser. Increase brightness when switching from bright-field to PlasDIC.• Swing analyzer module into the beam path or slide analyzer slider in beam path in intermediate plate.• Swing in the objective for PlasDIC.• Slide the slider PlasDIC on the nosepiece into the DIC slit of the used objective.• Adjust the contrast with the knurled screw of the slider PlasDIC. You can now see the structures inrelief or in pseudo dark-field. The best result is achieved in the relief mode.
