OPERATIONAxio Scope.A1 Illumination and Contrasting Method Carl ZeissM60-2-0007 e 05/08 874.1.9 Adjusting Reflected Light DIC and Reflected Light C-DIC(1) ApplicationThe reflected light DIC and the reflected light C-DIC methods (DIC = Differential Interference Contrast; C-DIC = Differential Interference Contrast in circular polarized light) are used for the high-contrast imagingof phase objects, i.e. objects which contrary to the amplitude objects, change predominantly the phase ofthe light.(2) Instrumentation− Axio Scope.A1 with adjusted halogen lamp HAL 100 mounted on the reflected light barrel− Objectives EC Epiplan-Neofluar, Epiplan with the additional label "DIC" or "Pol"− DIC slider, fitted to the objective engraved on the top surface of the slider with amplification andaperture or C-DIC slider 6x20 (in connection with the reflector module C DIC P&C, compensatorcompartment 6x20)(3) Reflected light DIC, preferably for objectives Epiplan 5x to 100x and LD-Epiplan 10x to 100x• Adjust the microscope for reflected light/bright-field as described in chapter 6 7 3 H 6 7 3 H 4.1.7. Open the fielddiaphragm enough so that the edge of the diaphragm lies just barely outside of the edge of the fieldof view in order to avoid reflexes.• Swing the reflector module C DIC P&C on the reflector turret/slider in the beam path.• Swing in objective position with DIC position on the nosepiece.• Slide the DIC slider in the compartment of the nosepiece (above the objective).• Put down the sample, focus and turn the mechanical stage so the structure of interest is visible withmaximum contrast.• The contrast can be optimized with the knurled button on the DIC slider.(4) Reflected light C-DIC• Adjust the microscope for reflected light/bright-field.• Switch reflector module C DIC P&C into thebeam path.• Slide the C-DIC slider (674H674HFig. 4-12/2) in thecompensator compartment 6x20 (675H675HFig. 4-12/1).• Put the sample down, focus and turn thecontrol knob (676H676HFig. 4-12/4) on the C-DIC slider sothe structure of interest is visible with maximumcontrast. No further stage rotation is necessary.• The contrast can be optimized by adjusting thecontrol knob (677H677HFig. 4-12/3) on the C-DIC slider.Fig. 4-12 Compensator compartment 6x20with C-DIC slider 6x20
