Zeiss LSM 880 NLO manuals
LSM 880 NLO
Table of contents
- Table Of Contents
- Table Of Contents
- Table Of Contents
- Table Of Contents
- Table Of Contents
- Table Of Contents
- Table Of Contents
- PURPOSE
- Backup
- HARDWARE ASPECTS
- Principle of Fluorescence Correlation Spectroscopy
- Principle of Airyscanning
- Optical Diagram of the LSM 880 (Schematic)
- Performance Features of the LSM 880
- Microscope Equipment of the LSM 880
- Computer Hardware and Software
- STARTUP AND SHUTDOWN OF THE SYSTEM
- Additional Software Tools
- Starting ZEN
- Shutdown Procedure
- Switching System Power Off
- INTRODUCTION TO THE SOFTWARE APPLICATION LAYOUT
- Introduction to ZEN
- Function Elements
- Application Bar
- File
- Maintain
- Camera
- Help – About
- Main Toolbar
- Status Bar
- Left Tool Area
- Tool Groups and Tools
- Context Menu of the Left Tool Area
- Center Screen Area
- Context Menu of the Center Screen Area
- Right Tool Area
- LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
- Locate Tab
- Microscope Control Tool for Axio Observer.Z1
- Microscope Control Tool for Axio Imager.Z2
- Controls for Axio Examiner
- Incubation Tool
- Acquisition Tab
- Configuration Functions
- Smart Setup
- Action Buttons
- Multidimensional Acquisition Settings
- Setup Manager – Laser
- Setup Manager – Experiment Designer
- Setup Manager – Imaging Setup
- Filter Cube Selection
- Stage Control
- Focus Control
- X-Cite Control
- Ratio Imaging
- Control Elements of the Imaging Setup Tool
- Setup Imaging with Airyscan Detector
- Acquisition Parameter – Acquisition Mode
- Scan Mode
- Averaging and Color Depth Selection
- HDR(High Dynamic Range)-Imaging
- Scan Area Definition
- Acquisition Mode Functions for LSM
- Acquisition Mode Settings for Airyscan
- Acquisition Parameter – Channels
- Defining Channels and Tracks
- Ratio Channels
- Using GaAsP Detectors and the temperature controlled side PMT for Photon Counting Imaging
- Channel Settings for Airyscan
- Acquisition Parameter – Focus
- Acquisition Parameter – Focus Devices and Strategy
- Acquisition Parameter – Stage
- Acquisition Parameter – Shuttle and Find
- Acquisition Parameter – Regions
- Multidimensional Acquisition – Z-Stack
- Performing a Z-Stack Using First/Last Mode
- Performing a Z-Stack Using Center Mode
- Performing a Z-Stack Using a Z-Piezo
- Optimize Sectioning and Step
- Correction
- Z-Stack Settings for Airyscan
- Multidimensional Acquisition – Tile Scan
- Scan Overview Image for Tiling
- Multidimensional Acquisition – Time Series
- Marker
- Start
- Position List
- Sample Carrier
- Setting Individual Offset Values for Positions in Position List Mode
- Scan Overview Image
- Multidimensional Acquisition – Auto Save
- Image Optimization
- Defining the Scan Parameters
- How to Optimize Image Quality
- How to Optimize Imaging with Airyscan
- Setup – Laser
- Light Path for 2 Channel Module (BiG.2 on DC out port)
- NDD Light Path (BiG.2 as NDD)
- Light Path for 32 Channel Module
- Selection of Laser Lines
- Pinhole Adjustment
- Setup – Acquisition
- Setup – Fit
- Defining a New Model
- Correlation Options
- Model Equations
- Definition of a Model
- Formula
- Setup – Correlator
- Navigation – Stage
- Navigation – Sample Carrier
- Navigation – Focus
- Multidimensional Acquisition Tools – Time Series
- Multidimensional Acquisition Tools – Positions
- Navigation – Incubation
- Multidimensional Acquisition Tools – Information on Experiment
- Action Buttons for FCS
- Method Tool
- Activation Tab Area
- Count Rate
- X-Y-Z Scan
- Snap
- Taking FCS Measurements
- Start and End of a Measurement
- Loading Stored Data Files
- FCS Correlation Diagram
- Image View Controls – Correlation Tab
- Image View Controls – Table Tab
- Saving Correlation Results
- Coincidence
- Information
- Processing Tab
- Processing – Airyscan Processing
- Processing – Maximum Intensity Projection
- Processing – Color-coded Projection
- Processing – Image Calculator
- Processing – Average
- Processing – Filter
- Processing – Linear Unmixing
- Processing – Ion Concentration
- Single Wavelength Dyes – Offline Calibration
- Ratiometric Dyes
- Processing – Correlation
- Processing – Modify Series
- Processing – HDR-imaging
- Processing – Stitch
- Processing – PALM
- PALM – Convert to Image
- PALM – Import PALM Molecules
- PALM – Localization Precision
- PALM – PALM
- PALM – Remove PALM Outliers
- Processing – Particle Tracking
- Processing – Experimental PSF
- Processing – Structured Illumination
- Structured Illumination – Fourier Transform
- Structured Illumination – Structured Illumination
- Structured Illumination – TIRF-Filtered
- Processing – Channel Alignment
- Processing – Lightsheet Processing
- Multiview Processing
- Online Multiview Processing
- Processing – Copy
- Processing – ICS
- Remove Structure
- ICS Correlation
- Map Filter
- NandB Analysis
- Processing – Adjust
- Interpolate Brightness and Contrast
- Channel Shift
- Shading Correction
- Maintain Tab
- Maintain – Adjust Pinhole and Collimators
- Pinholes Section Panel
- Collimators Section Panel
- How to Adjust the Pinhole and Collimator
- Maintain – Objectives
- Select Objective
- Add Objective
- Remove Objective
- Focus Speed
- Maintain – Filters
- Maintain – Airyscan
- Options – System Options
- System Options – Load Configurations
- System Options – Hardware
- System Options – Image Display
- Options – FCS Options
- FCS Options – Measure
- FCS Options – Auto Save
- CENTER SCREEN AREA / IMAGE CONTAINERS - DISPLAY AND IMAGE ANALYSIS
- Container Configuration with the Container Context Menu
- Dimensions
- Display
- Player
- Graphics
- Preview View
- Split View
- Gallery View
- Airyscan View
- Airyscan – View Option Tab Display
- Airyscan – View Option Tab Airyscan
- Ortho View
- Ortho – Select Function
- Ortho – Distance Function
- Cut View
- Shadow Projection
- Transparency Render Mode
- Maximum Mode
- Mixed Render Mode
- Clipping Planes
- Flying Mode
- Series
- Interactive Measurements
- Settings
- Options
- D View – Basic
- Histogram View
- Colocalization View
- How a Scatter Diagram is Generated
- Quantitative Colocalization Parameters Shown in the Data Table
- Overlap Coefficient, Overlap Coefficient after Manders
- Shuttle and Find View
- Mean of ROI: Additional View Type for Time Series
- Kinetic / FRAP View: Additional View Type for Time Series
- Lambda Coded: Additional View Types for Lambda Mode
- Unmixing View
- Automatic Component Extraction
- FRET View
- Raster Scanning Image Correlation Spectroscopy (RICS)
- Access to RICS
- Correlation Functionalities
- Display Functionalities
- Fitting Functionalities
- Model Functionalities
- Polarization Imaging
- Determination of G-factor
- Obtaining an Anisotropy Image
- Information View
- RIGHT TOOL AREA, DATA MANAGEMENT AND STORAGE
- ZEN File Browser
- Gallery View of the ZEN File Browser
- Form View of the ZEN File Browser
- Table View of the ZEN File Browser
- Images and Documents Panel
- Opening of Files via the "Open" Command in the File Menu
- Export of Images
- Index
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