CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 1555.2.19 Multidimensional Acquisition –Time SeriesWhen the Time Series tool tab is expanded theTime Series tool becomes available (Fig. 211). Inthe Time Series tool the parameters for thesequential acquisition of image frames (and linesfor the LSM image mode) as time series aredefined. Imaging in spot mode is only possible incombination with the settings of a time seriesacquisition in the LSM imaging mode. Theoptions in this mode within the Time Series toolare limited and explained below.To set up a simple time series acquisition it is enough to define the number of Cycles, which correspondsto the number of frames (or lines), imaged. This number can be defined with the Cycles slider or theCycles input box. A maximum value of 100,000 can be set. The amount of data that can be handled bythe system is limited. If the maximum amount is exceeded a warning will appear. In this case, it isnecessary to either change the destination for data storage and/or reduce the amount of cycles. The limitalso depends on the imaging setup and the acquisition mode. Note that it is an upper limit of handlingdata files, but this is beyond the value of 100 Gigabits.Interval defines a potential time interval, set in milliseconds (ms), seconds (sec) or minutes (min) by theInterval drop down menu, to separate the beginning of two cycles. A time shorter than the scan timefor a frame (or line) is not effective. The interval time can be changed by using the Interval slider orInterval input box, also while scanning.There are different advanced tools (only available in Show all mode) including− Interval Time− Marker− Start− End5.2.19.1 Interval TimeExpand the Interval field by clicking the triangle(Fig. 212). An Interval Time is a potential timeinterval between the end of one cycle and thebeginning of the next cycle. The time and time unitfor the delay time is set using the slider or inputbox of Interval Time. A cycle interval time is noteffective if a cycle delay time is defined, since theinterval time is always included in the delay. A totalof six different delay times can be defined byadding further lines clicking on the + button.Clicking the – button deletes the currentlyhighlighted delay time. Each delay time can becontrolled using the Trigger In function.(For a detailed description on the trigger use and interface see printed manual CHAPTER 4: ANNEX).Fig. 211 Time Series toolFig. 212 Time Series tool – Interval Time
