CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880184 000000-2071-464 10/2014 V_015.3.2.4 Selection of Laser Lines• Press the Laser button to display the lasercontrol expansion submenu (Fig. 265).Alternatively you have access to the Laser Linesin the Acquisition tab. They provide settingsfor:− Lasers for laser power used in measurementand pinhole adjustment,− Bleach for laser power used in bleachingSince the expansion submenu will close whenclicking in other areas, we recommend to use theLaser Lines from the Acquisition tab, if you wantto adjust the count rate.Lasers• Press the Visible Light or Invisible Light tab todisplay the laser control panel used formeasurements and pinhole adjustments (Lasersarea) or bleaching (Bleach area) (Fig. 266). Pressagain to close the panel.• Activate the lasers you want to use by checkingthe corresponding boxes. The lasers with sliderswill appear. Set the requested laser attenuation(%) using the sliders, the arrows, or typing inthe display field.Note, that lasers for measurements and(pre-)bleach can be selected independentlyfrom each other.If in AOTF Dampening a factor differentfrom 100 % was chosen, the set AOTF valuein Lasers and Bleach will be reduced by thatpercentage.Note, that lasers for measurements and pre-bleach can be selected independently fromeach other.The same options are available for the405 and 440 lasers and the NLO laser, ifavailable, in the Invisible Light laserselection box (Fig. 267).Fig. 265 Light Path tool with laser submenuFig. 266 Visible Light Laser panelFig. 267 Invisible Light Laser panel
