CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880384 000000-2071-464 10/2014 V_015.4.21 Processing – ICSLicensing Instrumentstandard optional LSM ELYRA Lightsheet Z.1Tool accepts exclusively input acquired with LSM 710 and LSM 780. See fulldescription of the tool set and section on RICS (Raster Scanning Image CorrelationSpectroscopy) for more details on requirements to be met.• Activate ICS in the Processing tool by clickingon the arrow.The ICS menu provides the following sub-processing functions: Detrend, ICS correlation,Map filter, NandB Analysis and Removestructures (Fig. 540).5.4.21.1 Detrend• The Detrend function is a high pass recursivefilter.• Click Detrend in the ICS tool. The MethodParameters menu will show the input optionsfor this function.• Select the desired image in the image container, then click Select to define this image as the Inputimage.The resuling output image using the time constant parameter is shown in the Preview tool and thePreview View tab in the image container.Input images are time series images.The Time constant used to detrend the image is the number of images it takes for the recursive filter todecay to 1/e. Use a smaller number for detrending faster moving larger objects like vesicles and a highernumber for detrending bleaching effect or slower cell movements.Fig. 540 Processing – ICS
