CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880330 000000-2071-464 10/2014 V_015.4.14.4 PALM – PALMThe PALM function allows setting filters for peakfinding and choosing a fit model for thelocalization calculations.Select the PALM tab to select the PALMProcessing tool (Fig. 431). Activate the image youwant to process in the image container and pressthe Select button to load the image. Then expandthe Method Parameters tool window to haveaccess to the Peak Finder and Localizer(Fig. 440).You have to select the subdirectory PALMtab in order to have the PALM functionalitiesavailable. The directory PALM tab will notgive access to the PALM tools.Pressing the Settings Default button will reset allfilter and parameter settings to the default values.Pressing the Settings Reuse button will recall theparameter setings of the actively selected image.The Channel drop down menu allows you in amulti-channel experiment to apply differentsettings to different channels, which are all listed(Fig. 441). If you select All, all channels will befitted the same way. If you select a channel, andchange settings, these setting will apply only tothis channel. Note, even if only one channel isselected, every channel will be fitted according tothe last settings.The Settings drop down menu (Fig. 442) willallow you to define how peak events will be fitted.You have three options:− Discard overlapping molecules (singleemitter): only single emitters will be takeninto account and fitted, whereas all multi-emitter events will be discarded.− Ignore overlap (ignore emitter): all single-and multi-emitter events will be taken intoaccount and fitted as single emitter events.Fig. 440 Processing – PALM – PALMFig. 441 Channel drop down menu of a dualchannel data setFig. 442 Settings drop down menu
